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RESEARCH PRODUCT

ODELAY: A Large-scale Method for Multi-parameter Quantification of Yeast Growth

Thurston HerricksJohn D. AitchisonSong LiFred D. Mast

subject

0301 basic medicinephenotype assayGeneral Chemical EngineeringSaccharomyces cerevisiaeGenetic FitnessSaccharomyces cerevisiaeyeastBiologylag timeGeneral Biochemistry Genetics and Molecular Biology03 medical and health sciencescarrying capacityGrowth rateMulti parameterMicroscopyGrowth rateGeneral Immunology and Microbiologybusiness.industryGeneral NeuroscienceScale (chemistry)biology.organism_classificationPhenotypeSolid mediumYeastBiotechnologyCellular BiologyPhenotype030104 developmental biologyIssue 125fitness assessmentBiological systembusiness

description

Growth phenotypes of microorganisms are a strong indicator of their underlying genetic fitness and can be segregated into 3 growth regimes: lag-phase, log-phase, and stationary-phase. Each growth phase can reveal different aspects of fitness that are related to various environmental and genetic conditions. High-resolution and quantitative measurements of all 3 phases of growth are generally difficult to obtain. Here we present a detailed method to characterize all 3 growth phases on solid media using an assay called One-cell Doubling Evaluation of Living Arrays of Yeast (ODELAY). ODELAY quantifies growth phenotypes of individual cells growing into colonies on solid media using time-lapse microscopy. This method can directly observe population heterogeneity with each growth parameter in genetically identical cells growing into colonies. This population heterogeneity offers a unique perspective for understanding genetic and epigenetic regulation, and responses to genetic and environmental perturbations. While the ODELAY method is demonstrated using yeast, it can be utilized on any colony forming microorganism that is visible by bright field microscopy.

yearjournalcountryeditionlanguage
2017-07-03Journal of Visualized Experiments
https://doi.org/10.3791/55879