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RESEARCH PRODUCT
The First Cell Division Cycle in Nicotiana Mesophyll Protoplasts Cultured in Vitro. I. Methods to Determine Cycle Kinetics
M. RösWolfgang Wernickesubject
Physiologymedicine.drug_classNicotiana tabacumPlant ScienceCell cycleBiologyProtoplastbiology.organism_classificationMonoclonal antibodyMolecular biologyIn vitrochemistry.chemical_compoundchemistryCell culturemedicineDAPIAgronomy and Crop ScienceNicotianadescription
Summary The progress of freshly isolated and in vitro cultured mesophyll protoplasts through the first mitotic cycle was studied with the aim of determining the frequency and timing of the G2-phase. Putative G1 and G2 phase cells were identified by measuring the relative DNA content through improved cytofluorimetry of DAPI-stained nuclei. S-phase nuclei were identified by labelling with the thymidine-analogue BrdU, which was subsequently immuno-localized with a BrdU-specific monoclonal antibody. The cells entered S-phase after 12–24h and a maximum of G2 cells was observed 24–36h after culture initiation. Most of the cells had divided after 48 to 72 h.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1991-06-01 | Journal of Plant Physiology |