6533b829fe1ef96bd1289920

RESEARCH PRODUCT

Uridine uptake inhibition as a cytotoxicity test for a human hepatoma cell line (HepG2 cells): comparison with the neutral red assay

Isabelle ValentinJean-claude LhuguenotMarie-christine ChagnonMurielle Philippe

subject

Neutral redCarcinoma Hepatocellular[SDV.TOX.TCA]Life Sciences [q-bio]/Toxicology/Toxicology and food chainToxicologyXenobiotics03 medical and health scienceschemistry.chemical_compoundInhibitory Concentration 500302 clinical medicineNeutral redToxicity TestsTumor Cells CulturedPotencyCytotoxic T cellHumansBenzopyrenesCytotoxicityColoring AgentsUridine030304 developmental biology0303 health sciencesReproducibility of ResultsMethylnitrosourea2-AcetylaminofluoreneUridine uptakeIn vitroUridineKineticschemistryBiochemistryCytotoxicity-helpG2 cell line[SDV.TOX.TCA] Life Sciences [q-bio]/Toxicology/Toxicology and food chain030220 oncology & carcinogenesisToxicityCarcinogensHepatocytesPyreneRNARegression AnalysisWater Pollutants Chemical

description

International audience; This study describes a sensitive microassay for measuring cytotoxicity based on the degree of inhibition of RNA synthesis in HepG2 cells. RNA synthesis is measured by the kinetic uptake of radiolabeled uridine. A large number of compounds were tested in a wide range of concentrations. The concentration required to induce 50% inhibition of HepG2 uridine uptake rates (IC50) was determined for each compound and used to rank its potency. These IC50s were compared with IC50s measured with the neutral red assay. 2-acetylaminofluorene, benzo[a]pyrene and methylnitrosourea were not cytotoxic in the neutral red assay. Uridine uptake was always inhibited at lower concentrations than those required in the neutral red assay, suggesting that the uridine uptake assay is a more sensitive indicator of toxic action than the neutral red inclusion. Uridine uptake assay provides a rapid and quantitative method for assessing toxicity in a human cell line. Application of this method to bottled spring waters are described. Due to its high sensitivity and reproducibility, this method provides a suitable tool for screening a great number of samples and will be a helpful test for evaluating food safety and controlling the recycling process of wrapping materials.

https://hal-agrosup-dijon.archives-ouvertes.fr/hal-01981819