6533b829fe1ef96bd1289b50

RESEARCH PRODUCT

Cytochrome oxidase activity in the mitochondria of unfertilized and fertilized sea urchin eggs

Rachele Maggio

subject

biologyZygoteCytochrome cCell BiologyMitochondrionbiology.organism_classificationBlastulaParacentrotus lividusMitochondriaElectron Transport Complex IVHuman fertilizationCytochrome oxidase activityBiochemistrySea Urchinsbiology.animalembryonic structuresbiology.proteinAnimalsCytochrome c oxidaseOxidoreductasesSea urchinOvum

description

Abstract The cytochrome oxidase activity of isolated mitochondria of unfertilized and fertilized eggs of Paracentrotus lividus has been studied. It has been found that whereas in the mitochondria of unfertilized eggs saturation is reached at a cytochrome c concentration of 3.28 × 10−5 M, in those of fertilized eggs this occurs at a concentration of 5.74 × 10−5 M. It is shown that upon fertilization an increase of about 30 per cent of the activity of cytochrome oxidase takes place and no further changes appear to occur until blastula stage.

https://doi.org/10.1016/0014-4827(59)90254-x