6533b829fe1ef96bd128a3c4
RESEARCH PRODUCT
Immunohistological and immunoelectron microscopic identification of TNF alpha in normal human and murine epidermis.
Jürgen KnopH. MeyerK. Schulze-osthoffGerhard Koldesubject
AdultMalePathologymedicine.medical_specialtyImmunoelectron microscopymedicine.medical_treatmentDermatologyMicemedicineAnimalsHumansMicroscopy ImmunoelectronCellular localizationMice Inbred BALB Cintegumentary systemEpidermis (botany)biologyTumor Necrosis Factor-alphaGeneral MedicineMiddle AgedMolecular biologyImmunohistochemistryCytokinemedicine.anatomical_structurePolyclonal antibodiesbiology.proteinImmunohistochemistryFemaleEpidermisMerkel cellImmunostainingdescription
The presence, distribution and cellular localization of tumour necrosis factor-alpha (TNF alpha) were investigated in normal human and murine epidermis using immunohistological and immunoelectron microscopic methods with monoclonal and polyclonal antibodies. The immunostaining revealed an intercellular plasma membrane and cytoplasmic labelling of the epidermal keratinocytes, but no labelling of Langerhans cells, melanocytes and Merkel cells. Large amounts of TNF alpha were regularly found in the sebaceous glands. These findings demonstrate that epidermal keratinocytes and especially sebocytes produce and release TNF alpha and that this keratinocyte-derived cytokine may be important for the structural and functional homeostasis of normal epidermis.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1992-06-01 | Archives of dermatological research |