6533b829fe1ef96bd128a4fa
RESEARCH PRODUCT
Delta 5-desaturation of dihomogammalinolenic acid (20:3(n-6)) into arachidonic acid (20:4(n-6)) by rat liver microsomes and incorporation of fatty acids in microsome phospholipids.
Jean Paul BlondJean Bézardsubject
Fatty Acid DesaturasesMaleBiophysicsPhospholipidArachidonic AcidsBiochemistrySubstrate Specificitychemistry.chemical_compoundEndocrinology81114-Eicosatrienoic AcidEssential fatty acidAnimalsIncubationPhospholipidschemistry.chemical_classificationbiologyFatty Acids EssentialSubstrate (chemistry)Rats Inbred Strainsbiology.organism_classificationDietary FatsRatsKineticsMicrosomachemistryBiochemistryMicrosomeMicrosomes LiverArachidonic acidPolyunsaturated fatty aciddescription
Abstract Liver microsomes of rats fed an essential fatty acid (EFA)-deficient diet or a commercial balanced diet were used to study the effect of incubation time on the Δ 5 -desaturation of [ 14 C] dihomogammalinolenic acid (20:3( n −6)) into arachidonic acid (20:4( n −6)) and incorporation of the two acids into microsomal phospholipids. The EFA-deficient diet highly increased the desaturation rate of 20:3( n −6). Incorporation of the formed 20:4( n −6) into microsomal phospholipids was also increased but at saturating concentration of substrate only. At early times of incubation, the precursor 20:3( n −6) was rapidly incorporated into phospholipids. Formation and incorporation of 20:4( n −6) into phospholipids proceeded more progressively. Data suggest that desaturation of 20:3( n −6) and incorporation of both 20:3( n −6) and 20:4( n −6) into phospholipids occur concomitantly and independently.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1991-07-30 | Biochimica et biophysica acta |