Hloramfenikola selektīvā spiediena ietekme uz acetiltransferāzes gēna aktivēšanu pAdCat plazmīdā Escherichi coli kultūras stacionārā fāzē

6533b829fe1ef96bd128a770

RESEARCH PRODUCT

Hloramfenikola selektīvā spiediena ietekme uz acetiltransferāzes gēna aktivēšanu pAdCat plazmīdā Escherichi coli kultūras stacionārā fāzē

Kristīne Gruzdeva

subject

stacionārā augšanas fāze pAdCat IS10 cat selektīvais spiediens Bioloģija

description

IS10 ir viens no aktīvākajiem transpozoniem, kas nodrošina adapatīvo mutaģenēzi baktērijām, aktivizējot neekspresējošus gēnus. Darba mērķis bija noskaidrot hloramfenikola selektīvā spiediena ietekmi uz IS10 elementa spēju efektīvāk aktivizēt bezpromotera acetiltransferāzes (cat) gēnu pAdCat plazmīdā Escherichia coli kultūras stacionārā augšanas fāzē. Baktēriju celmi tika transformēti ar pAdCat plazmīdu, kas kalpo par vektoru-lamatu IS10 elementu izolēšanai pēc pozitīvās selekcijas principa. Šūnām, atrodoties trīs dienas stacionārajā fāzē, novēroja IS10 transpozīciju un bezpromotera hloramfenikola acetiltransferāzes (cat) gēna aktivizēšanu. Gēna aktivizēšanu nodrošināja gan IS10R, gan IS10H. Sekvenēšanas un reālā laika PCR rezultāti parādīja, ka hloramfenikola selektīvā spiediena ietekmē IS10 transpozēja tuvāk cat apgrieztā orientācija, piešķirot šūnām izteiktu hloramfenikola rezistences fenotipu ar augstu cat ekspresiju.

year	journal	country	edition	language
2015-01-01

https://dspace.lu.lv/dspace/handle/7/28562

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