6533b829fe1ef96bd128aa50

RESEARCH PRODUCT

Nouvelles stratégies de stabilisation des bactéries extrêmement sensibles à l’oxygène : cas du probiotique Faecalibacterium prausnitzii.

subject

description

Intestinal bacteria are of growing interest in the areas of health and wellness [1, 3]. The intestinal ecosystem is characterized by its complex composi-tion and its very low oxygen content. Therefore, nu-merous bacterial species isolated from this environ-ment are extremely sensitive to oxygen. This feature is responsible for the difficulty of implementation of these microorganisms for probiotic large-scale appli-cations, particularly during processing and storage. In this presentation, we present the main steps that have been overcome to conserve and design a stabilization strategy for the bacteria Faecalibacterium prausnitzii, recognized for their anti-inflammatory activities intestinal and their beneficial effects on Crohn’s disease and ulcerative colitis [3]. The first objective was to look for a medium and conditions optimized to stabilize the bacteria to the dry state in powder form, with a high survival rate (FUI FPARIS). Indeed, stabiliza-tion processes by dehydration lead to mechanical and oxidative perturbations [4] affecting survival and functionality of cells [5]. The second objective was to develop a specific protection protocol for the conservation of the probiotic in a dry solid form (capsules or microgranules) and to maintain vi-ability during passage through the gastrointestinal tract. Encapsulation has been considered since this technique protects molecules or cells against various environmental perturbations [6-8]...