6533b829fe1ef96bd128acb2
RESEARCH PRODUCT
Stain-free virtual histology of Bombus terrestris compound eyes by laboratory phase-contrast nano-CT
Jenny RomellVun Wen JieHans M. HertzArttu MiettinenArttu MiettinenArttu MiettinenEmily Bairdsubject
Basement membranePathologymedicine.medical_specialtygenetic structuresbiologyChemistryHistologybiology.organism_classificationStaineye diseaseslaw.inventionStainingmedicine.anatomical_structurelawBombus terrestrisCorneaMicroscopymedicinesense organsElectron microscopedescription
The visual systems of pollinating insects are studied to understand the effects of a changing environment, and are to date imaged using microscopy or micro-CT. Microscopy only allows two-dimensional imaging and conventional micro-CT requires heavy-metal staining of the samples. Here we present virtual histology of compound eyes of bumblebee (Bombus terrestris) using a laboratory nano-CT system. Propagation-based phase-contrast CT allows three-dimensional imaging of samples without using any staining. Micrometre-resolution images of the microanatomy of the eyes were reconstructed, and the features identified in CT (cornea, crystalline cones, pigment, photoreceptor cells, basement membrane) were confirmed with electron microscopy.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2021-08-01 | Developments in X-Ray Tomography XIII |