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RESEARCH PRODUCT
Liquid/Gas and Liquid/Liquid Phase Equilibria of the System Water/Bovine Serum Albumin
Bernhard A. WolfRei SugayaYurij A. AntonovJohn Eckeltsubject
chemistry.chemical_classificationChromatography GasChemistrySpinodal decompositionVapor pressureLiquid gasAnalytical chemistryWaterSerum Albumin BovinePolymerPolarizerSurfaces Coatings and Filmslaw.inventionOptical microscopelawPhase (matter)Materials ChemistryAnimalsThermodynamicsCattleGas chromatographyPhysical and Theoretical Chemistrydescription
The thermodynamic behavior of the system H2O/BSA was studied at 25 °C within the entire composition range: vapor pressure measurements via head space sampling gas chromatography demonstrate that the attainment of equilibria takes more than one week. A miscibility gap was detected via turbidity and the coexisting phases were analyzed. At 6 °C the two phase region extends from ca. 34 to 40 wt % BSA; it shrinks upon heating. The polymer rich phase is locally ordered, as can be seen under the optical microscope using crossed polarizers. The Flory-Huggins theory turns out to be inappropriate for the modeling of experimental results. A phenomenological expression is employed which uses three adjustable parameters and describes the vapor pressures quantitatively; it also forecasts the existence of a miscibility gap.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2013-04-13 | The Journal of Physical Chemistry B |