Ekstracelulārās kalcija koncentrācijas ietekme uz krūts vēža šūnu līnijas MCF-7 migrāciju un invāziju

6533b82afe1ef96bd128b25a

RESEARCH PRODUCT

Ekstracelulārās kalcija koncentrācijas ietekme uz krūts vēža šūnu līnijas MCF-7 migrāciju un invāziju

subject

krūts vēzis Bioloģija invāzija MCF-7 ekstracelulārā Ca2+ koncentrācija migrācija

description

Visbiežākais ar krūts vēzi saistītais nāves iemesls ir metastāzes citos orgānos. Lai metastāzes veidotos, šūnām ir jābūt spējīgām migrēt un invadēt. Tomēr pētījumu rezultāti par ekstracelulārā Ca2+ ietekmi uz krūts vēža šūnu spēju migrēt un invadēt ir pretrunīgi. Darba mērķis bija noteikt ekstracelulārā Ca2+ koncentrācijas ietekmi uz krūts vēža šūnu līnijas MCF-7 migrāciju un invāziju. Šūnas tika kultivētas barotnēs ar dažādu Ca2+ koncentrāciju. Tika konstatēts, ka palielināta ekstracelulārā Ca2+ koncentrācija barotnē samazina MCF-7 šūnu spēju migrēt un invadēt, bet neietekmē populācijas dubultošanās laiku. Šūnās, kas kultivētas barotnēs ar palielinātu Ca2+ koncentrāciju, ciešo starpšūnu kontaktu komponenta gēna CLDN3 ekspresija bija palielināta. Darbs izstrādāts RSU Onkoloģijas institūta Molekulārās ģenētikas laboratorijā no 2017. gada novembra līdz 2018. gada maijam.

year	journal	country	edition	language
2018-01-01

https://dspace.lu.lv/dspace/handle/7/39651

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