6533b82afe1ef96bd128b869
RESEARCH PRODUCT
Molecular diversity and growth features of Flavobacterium columnare strains isolated in Finland
E. T. ValtonenV. Hirvelä-koskiH. KunttuL.-r. SuomalainenMarja Tiirolasubject
Molecular Sequence DataMicrobial Sensitivity TestsSodium ChlorideAquatic ScienceBiologyFlavobacteriumMicrobiologylaw.inventionFish DiseasesIntergenic regionFlavobacteriaceae InfectionslawRNA Ribosomal 16SDNA Ribosomal SpacerAnimalsFinlandPhylogenyEcology Evolution Behavior and SystematicsPolymerase chain reactionTemperatureGenetic VariationGenomovarHydrogen-Ion ConcentrationRibosomal RNAbiology.organism_classification16S ribosomal RNAFlavobacteriaceaeFlavobacterium columnareRestriction fragment length polymorphismdescription
ABSTRACT: Columnaris disease caused by Flavobacterium columnare is a problem in fish farmingworldwide. During the last 15 yr, outbreaks have started to emerge in Finland. Flavobacteriumcolumnare Type Strain NCIMB 2248 T and 30 Finnish F. columnare isolates were studied usinganalysis of 16S rDNA by restriction-fragment length polymorphism (16S RFLP), length heterogeneityanalysis of polymerase chain reaction (LH-PCR) products, automated ribosomal intergenic spaceranalysis (ARISA), and 16S rDNA sequence analysis. All isolates fell into RFLP Genomovar I and hadthe same length in the LH-PCR analysis. Based on ARISA, 8 genetically different strains wereselected for further analyses. The growth of these strains under different temperatures, NaClconcentrations, and pH values was tested. The Finnish F. columnare strains did not grow at NaClconcentrations >0.1% or at pH values ≤6.5, and they were susceptible to several antimicrobial agents,but not to Polymyxin B or neomycin. These findings may aid in development of methods for diseasemanagement at fish farms.KEY WORDS:
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2006-08-01 | Diseases of Aquatic Organisms |