6533b82afe1ef96bd128b9d3
RESEARCH PRODUCT
Investigations on the Mechanism of Induction of the Alkaline Phosphatase by Bromodesoxyuridine in Herpes simplex Virus Transformed Cells and the Transport of Uridine
Falke DSchneider Dsubject
PrednisoloneHamsterCycloheximidemedicine.disease_causeGeneral Biochemistry Genetics and Molecular Biologychemistry.chemical_compoundCricetinaemedicineAnimalsSimplexvirusCycloheximideUridineCells CulturedBiological TransportEmbryoAlkaline PhosphataseCell Transformation ViralEmbryo MammalianUridineKineticsHerpes simplex virusBromodeoxyuridinechemistryBiochemistryEnzyme InductionPhosphorylationAlkaline phosphataseCytosinedescription
Addition of BrdUrd in combination with prednisolone to HSVi-transformed hamster embryo cells induces an alkaline phosphatase (AP). FdUrd enhances, dThd reduces the inducing capacity of BrdUrd and prednisolone. Induction is prevented by addition of cycloheximide or of cytosine arabinoside. BrdUrd reduces transport and phosphorylation of exogenously applied labeled Urd, especially the amount of UTP, UDP and Urd-diphosphate sugars. The Lineweaver-Burk-plot of uridine-uptake after addition of BrdUrd reveales the characteristics of the mixed type inhibition (competitive-noncompetitive).
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1980-11-01 | Zeitschrift für Naturforschung C |