6533b82afe1ef96bd128ba3e
RESEARCH PRODUCT
Evidence for a geranyl-diphosphate synthase located within the plastids of Vitis vinifera L. cultivated in vitro
Marc ClastreR DargentC. AmbidE. SolerGilles FeronM. Gleizessubject
0106 biological sciencesMonoterpenePlant Science01 natural sciences[SDV.GEN.GPL]Life Sciences [q-bio]/Genetics/Plants genetics03 medical and health sciences[SDV.GEN.GPL] Life Sciences [q-bio]/Genetics/Plants geneticsOrganelleGeneticsPlastidComputingMilieux_MISCELLANEOUS030304 developmental biologychemistry.chemical_classification0303 health sciencesATP synthasebiologyMetabolismTECHNIQUE DES TRACEURSTerpenoidEnzymeBiochemistrychemistryCULTURE DE CELLULECell culturebiology.protein010606 plant biology & botanydescription
Intact plastids from cell suspensions of Vitis vinifera L. cv. Muscat de Frontignan, free of detectable contamination by other particles as judged by the distribution of organelle-specific marker enzymes and by electron microscopy, exhibit geranyl-diphosphate synthase activity (EC 2.5.1.1). This synthase activity remains stable after tryptic digestion of unlysed organelles and is enhanced by plastid disruption. We conclude that the enzyme is located within the organelle. The possibility of an isopentenyl diphosphate/dimethylallyl diphosphate translocating system which would play a major role in the regulation of monoterpene metabolism is discussed.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1992-01-01 | Planta |