Purīnu auksotrofijas ietekme uz maizes rauga S. cerevisiae subletālo stresu izturību

6533b82afe1ef96bd128bc62

RESEARCH PRODUCT

Purīnu auksotrofijas ietekme uz maizes rauga S. cerevisiae subletālo stresu izturību

subject

purīnis badināšana karstuma stress acetāta stress oksidatīvais stress Bioloģija

description

Darbā tika pārbaudīta maizes rauga stresa izturība purīnu trūkuma gadījumā. Ietekme uz izdzīvotību tika novērtēta audzējot gan auksotrofu, gan prototrofa celmu šūnas barotnēs ar atšķirīgu oglekļa avotu un purīnu sastāvu, pēc tam visus paraugus pakļaujot subletālam oksidatīvajam, termiskajam vai vāju skābju stresam stresam un novērtējot izdzīvotību. Darbā konstatēts, ka adenīna trūkums izraisa visu trīs pētīto stresu izturības pieaugumu. Oksidatīvā stresa izturības pieaugums var tikt izskaidrots ar elpošanas pieaugumu purīna trūkuma gadījumā, bet termiskā un vāju skābju stresa izturība nepieaug pie oksidatīva oglekļa avota, bet to ietekmē guanīna klātbūtne. Rezultāti ļauj secināt, ka purīnu badošanās izraisītā stresa izturība nav izskaidrojama tikai ar šūnu arestu vai palielinātu elpošanu, bet arī signālceļu iesaisti izturības fenomenā.

year	journal	country	edition	language
2016-01-01

https://dspace.lu.lv/dspace/handle/7/32206

PEPhysical Sciences and Engineering
- PE2Fundamental Constituents of Matter
  - PE2_1Theory of fundamental interactions
  - PE2_2 Phenomenology of fundamental interactions
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  - PE2_10Atomic, molecular physics
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- PE1Mathematics
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