6533b82afe1ef96bd128c13c

RESEARCH PRODUCT

A new method for the in situ determination of phospholipids after thin-layer separation

H.w. PeterH.u. Wolf

subject

Differential centrifugationChromatographybiologyChemistryATPaseOrganic ChemistryPhospholipidGeneral MedicineFibrilBiochemistryAnalytical ChemistryCalcium ATPasechemistry.chemical_compoundIonic strengthbiology.proteinlipids (amino acids peptides and proteins)Composition (visual arts)Na+/K+-ATPase

description

Abstract A very sensitive method has been devised for the in situ determination of phospholipids after thin-layer chromatographic separation, which enabled us to investigate the phospholipid content of erythrocytes and ATPase preparations. The phospholipid compositions of the ATPase preparations and of the erythrocytes are different, and the relative phospholipid compositions of the Na,K-ATPase preparations are also different, which indicates that Na,K- and Ca-ATPase seem to be different with regard to their phospholipid composition. An increase in temperature during the preparation procedure yields a Ca-ATPase preparation (II), which exhibits different kinetic properties and a different phospholipid composition from those of the normal Ca-ATPase preparation (I). By changing the ionic strength and performing density gradient centrifugation, preparation (I) yields three fractions, one of which is composed of fibrils. The phospholipid composition of this fraction is approximately the same as that of preparation (II), and it contains ATPase activity and might possibly constitute one of the Ca-ATPase systems.

yearjournalcountryeditionlanguage
1973-08-01Journal of Chromatography A
https://doi.org/10.1016/s0021-9673(01)80071-6