6533b82afe1ef96bd128c160
RESEARCH PRODUCT
Reaction of zearalenone and α-zearalenol with allyl isothiocyanate, characterization of reaction products, their bioaccessibility and bioavailability in vitro.
Fernando Bittencourt LucianoFederica SaladinoKeliani BordinC. Fernández-blancoG. MecaJ. MañesMaría-josé RuizMónica Fernández-franzónsubject
0301 basic medicineBiological AvailabilityAnalytical Chemistry03 medical and health scienceschemistry.chemical_compound0404 agricultural biotechnologyIsothiocyanatesChemical reductionOrganic chemistryHumansFood scienceEstrogens Non-SteroidalMycotoxinCytotoxicityZearalenonefood and beverages04 agricultural and veterinary sciencesGeneral MedicineMycotoxinsAllyl isothiocyanate040401 food scienceIn vitroBioavailability030104 developmental biologychemistryToxicityZearalenoneZeranolCaco-2 CellsFood Sciencedescription
This study investigates the reduction of zearalenone (ZEA) and α-zearalenol (α-ZOL) on a solution model using allyl isothiocyanate (AITC) and also determines the bioaccessibility and bioavailability of the reaction products isolated and identified by MS-LIT. Mycotoxin reductions were dose-dependent, and ZEA levels decreased more than α-ZOL, ranging from 0.2 to 96.9% and 0 to 89.5% respectively, with no difference (p⩽0.05) between pH 4 and 7. Overall, simulated gastric bioaccessibility was higher than duodenal bioaccessibility for both mycotoxins and mycotoxin-AITC conjugates, with duodenal fractions representing ⩾63.5% of the original concentration. Simulated bioavailability of reaction products (α-ZOL/ZEA-AITC) were lower than 42.13%, but significantly higher than the original mycotoxins. The cytotoxicity of α-ZOL and ZEA in Caco-2/TC7 cells was also evaluated, with toxic effects observed at higher levels than 75μM. Further studies should be performed to evaluate the toxicity and estrogenic effect of α-ZOL/ZEA-AITC.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2017-02-01 | Food chemistry |