6533b82afe1ef96bd128c164

RESEARCH PRODUCT

Artificial chromosome libraries of Streptomyces coelicolor A3(2) and Planobispora rosea

Rosa AlduinaStefano DonadioMargherita SosioSimona De GraziaLuca DolceAnna Maria PugliaPaola Salerno

subject

GeneticsQuality ControlBacterial artificial chromosomeChromosomes Artificial BacterialChromosomes Artificial Bacterial; Molecular Biology; Quality Control; Streptomyces; Gene LibrarybiologyStreptomyces coelicolorbiology.organism_classificationMicrobiologyStreptomycesGenomeInsert (molecular biology)StreptomycesShuttle vectorStreptomyceGeneticsGenomic libraryActinomycetalesMolecular BiologyGene Library

description

Using an Escherichia coli-Streptomyces shuttle vector derived from a bacterial artificial chromosome (BAC), we developed methodologies for the construction of BAC libraries of filamentous actinomycetes. Libraries of Streptomyces coelicolor, the model actinomycete, and Planobispora rosea, a genetically intractable strain, were constructed. Both libraries have an average insert size of 60 kb, with maximal insert larger than 150 kb. The S. coelicolor library was evaluated by selected hybridisations to DraI fragments and by end sequencing of a few clones. Hybridisation of the P. rosea library to selected probes indicates a good representation of the P. rosea genome and that the library can be used to facilitate the genomic analysis of this actinomycete.

yearjournalcountryeditionlanguage
2003-01-01
http://hdl.handle.net/10447/286039