HBV kapsīdu savākšanās inhibīcija ar mazmolekulāriem savienojumiem

6533b82afe1ef96bd128c5f1

RESEARCH PRODUCT

HBV kapsīdu savākšanās inhibīcija ar mazmolekulāriem savienojumiem

Unda Nelda Dubova

subject

BHK-21 HBc Nukleokapsīdu inhibitori Bioloģija Bay-41-4109 SFV1

description

Hepatīta B vīrusa (HBV) infekcija hroniskā formā ir viena no bīstamākajām mūsdienu saslimšanām, tāpēc aktīvi tiek meklētas iedarbīgas zāles infekcijas ārstēšanai. Ar mērķi atrast efektīvu un netoksisku HBV nukleokapsīdu inhibitoru, ir veikta plaši pētīta Bay-41-4109 inhibitora un tā analogu pārbaude zīdītāju šūnās. Šajā darbā HBV kapsīdas proteīna (HBc) sintēze BHK-21 šūnās nodrošināta, izmantojot rekombinantu alfavīrusa replikonu. Ir pārbaudīta Bay-41-4109 un tā analogu citotoksicitāte šūnās. Izmantojot natīvās agarozes un SDS-PAA gēla elektroforēzes un imunoblota metodes tika salīdzināta inhibitoru ietekme uz HBc proteīna ekspresiju un nukleokapsīdu veidošanos šūnās. Atrasts, ka viens no pētītiem inhibitoriem inducēja HBc agregātu veidošanos un ir nepieciešami turpmāki pētījumi tā ietekmes uz vīrusa replikāciju noskaidrošanai.

year	journal	country	edition	language
2016-01-01

https://dspace.lu.lv/dspace/handle/7/33182

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