Mitohondriālo slimību diagnostika, izmantojot molekulārās bioloģijas metodes un citoplazmatisko hibrīdu šūnu modeļus

6533b82afe1ef96bd128c69e

RESEARCH PRODUCT

Mitohondriālo slimību diagnostika, izmantojot molekulārās bioloģijas metodes un citoplazmatisko hibrīdu šūnu modeļus

subject

mtDNS kopiju skaits OXPHOS kompleksi mitohondriālās slimības Bioloģija ATF

description

Mitohondriju galvenā funkcija ir enerģijas ražošana šūnā. Traucējumi mitohondriju elpošanas ķēdē var izraisīt plaša spektra patoloģijas. Patogēno mtDNS mutāciju ietekmi uz šūnu fenotipu iespējams pētīt citoplazmatisko hibrīdu šūnu līnijās. Darba mērķis ir analizēt citoplazmatisko hibrīdu šūnu modeļus un noteikt to piemērotību mitohondriālo slimību raksturošanai un diagnostikai. Tika veikta kontroles grupas un pacientu citoplazmatisko hibrīdu šūnu līniju mtDNS fragmentu sekvenēšana, mtDNS kopiju skaita, ATF koncentrācijas un elpošanas ķēdes kompleksu aktivitāšu noteikšana un salīdzināšana, kā arī tika raksturota izveidoto šūnu līniju stabilitāte. Lai apstiprinātu atbilstību donoru trombocītu mtDNS, tika veikta mtDNS fragmentu sekvenēšana 28 kontroles grupas šūnu līnijām un 12 pacientu šūnu līnijām. Tika noteikts mtDNS kopiju skaits 38 kontroles un 7 pacientu šūnu līnijām. ATF koncentrācija tika analizēta 7 kontroles un 8 pacientu līnijās. Elpošanas ķēdes kompleksu aktivitāte tika pārbaudīta divās šūnu līnijās un salīdzināta ar laboratorijā iepriekš veiktajiem mērījumiem. Citoplazmatisko hibrīdu šūnu modeļu stabilitātes raksturošanai, iepriekš minētie mērījumi tika veikti un salīdzināti starp vienas līnijas dažādu pasāžu šūnām.

year	journal	country	edition	language
2017-01-01

https://dspace.lu.lv/dspace/handle/7/36196

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