6533b82bfe1ef96bd128d71a

RESEARCH PRODUCT

Extracellular Vesicles from Healthy Cells Improves Cell Function and Stemness in Premature Senescent Stem Cells by miR-302b and HIF-1α Activation.

Jose ViñaLucia Gimeno-mallenchAurora Román-domínguezCristina Mas BarguesConsuelo BorrásJorge Sanz-rosMarta Inglés

subject

AdultMale0301 basic medicineCell typephysiological oxygen concentrationsenescenceAdolescentphysioxialcsh:QR1-502Biochemistrylcsh:MicrobiologyArticleKruppel-Like Factor 4Young Adult03 medical and health sciences0302 clinical medicineSOX2HumansMolecular BiologyCells CulturedDental PulpTissue homeostasisChemistryStem CellsMesenchymal stem cellagingHypoxia-Inducible Factor 1 alpha Subunit3. Good healthOxygen tensionCell biologyMicroRNAs030104 developmental biologyKLF4030220 oncology & carcinogenesisredoxFemaleFisiologia humanaStem cellextracellular vesiclesoxygenIntracellular

description

Aging is accompanied by the accumulation of senescent cells that alter intercellular communication, thereby impairing tissue homeostasis and reducing organ regenerative potential. Recently, the administration of mesenchymal stem cells (MSC)-derived extracellular vesicles has proven to be more effective and less challenging than current stem cell-based therapies. Extracellular vesicles (EVs) contain a cell-specific cargo of proteins, lipids and nucleic acids that are released and taken up by probably all cell types, thereby inducing functional changes via the horizontal transfer of their cargo. Here, we describe the beneficial properties of extracellular vesicles derived from non-senescent MSC, cultured in a low physiological oxygen tension (3%) microenvironment into prematurely senescent MSC, cultured in a hyperoxic ambient (usual oxygen culture conditions, i.e., 21%). We observed that senescent MCS, treated with EVs from non-senescent MCS, showed reduced SA-&beta

yearjournalcountryeditionlanguage
2020-06-25
10.3390/biom10060957https://hdl.handle.net/10550/76453