6533b82bfe1ef96bd128d780
RESEARCH PRODUCT
Molecular cloning ofTrichophyton mentagrophytes DNA sequences with promoter activity inEscherichia coli
Daniel RamónC. TortajadaSergi Ferrersubject
GeneticsPhysiologyNucleic acid sequenceGeneral MedicineMolecular cloningBiologymedicine.disease_causeApplied Microbiology and BiotechnologyMolecular biologyDNA sequencinggenomic DNAchemistry.chemical_compoundPlasmidchemistrymedicineEscherichia coliDNABiotechnologySouthern blotdescription
A promoter probe library from the dermatophyte fungusTrichophyton mentagrophytes has been constructed in the pVB32 plasmid vector. Using this library, a set ofT. mentagrophytes DNA sequences with promoter activity inEscherichia coli has been cloned. The size and the resistance phenotype conferred by these DNA fragments varied. Southern blot analysis confirmed that they were derived fromT. mentagrophytes genomic DNA.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1992-03-01 | World Journal of Microbiology and Biotechnology |