6533b82bfe1ef96bd128e308

RESEARCH PRODUCT

Glycopeptide-functionalized gold nanoparticles for antibody induction against the tumor associated mucin-1 glycoprotein

Ulrika WesterlindHorst KunzRoberto FiammengoBastian GerlitzkiFederica DegliangeliHui CaiEdgar SchmittBjörn Palitzsch

subject

Genes MHC Class IIMolecular Sequence DataClinical BiochemistryEpitopes T-LymphocyteMetal NanoparticlesPharmaceutical Science02 engineering and technology010402 general chemistryCancer Vaccines01 natural sciencesBiochemistryAntibodiesEpitopeMiceImmune systemNeoplasmsDrug DiscoveryAnimalsHumansAmino Acid Sequenceskin and connective tissue diseasesMolecular BiologyMUC1Cancerchemistry.chemical_classificationAntiserumVaccinesbiologyMucin-1Organic ChemistryGlycopeptides021001 nanoscience & nanotechnologyMolecular biologyGlycopeptide0104 chemical scienceschemistryColloidal goldImmunologyMCF-7 Cellsbiology.proteinNanoparticlesMolecular MedicineImmunizationGoldAntibody0210 nano-technologyGlycoprotein

description

We report the preparation of gold nanoparticle (AuNP)-based vaccine candidates against the tumor-associated form of the mucin-1 (MUC1) glycoprotein. Chimeric peptides, consisting of a glycopeptide sequence derived from MUC1 and the T-cell epitope P30 sequence were immobilized on PEGylated AuNPs and the ability to induce selective antibodies in vivo was investigated. After immunization, mice showed significant MHC-II mediated immune responses and their antisera recognized human MCF-7 breast cancer cells. Nanoparticles designed according to this report may become key players in the development of anticancer vaccines.

yearjournalcountryeditionlanguage
2015-09-25Bioorganic & Medicinal Chemistry
https://doi.org/10.1016/j.bmc.2016.01.044