6533b82cfe1ef96bd128ea57

RESEARCH PRODUCT

Effects of Atropine on Acetylcholine Overflow from Perfused Chicken Hearts

C. KriegH. Kilbinger

subject

AtropineChromatographySalivary secretionChemistryDimethylphenylpiperaziniumSmall intestinal motilityExtraction (chemistry)medicineStimulationPerfusionAcetylcholinemedicine.drug

description

Isolated chicken hearts were perfused (20 ml/min) with Tyrode’s solution. Release of acetylcholine (ACh) was evoked either by electrical stimulation (1 ms; 15 mA) of both preganglionic vagus nerves or by perfusion with dimethylphenylpiperazinium (DMPP). ACh was extracted from the perfusates by ion-pair extraction and determined by gas chromatography.

https://doi.org/10.1007/978-3-662-39532-5_217