Cytotoxicity and induction of DNA double-strand breaks by components leached from dental composites in primary human gingival fibroblasts

6533b82cfe1ef96bd128ecb8

RESEARCH PRODUCT

Cytotoxicity and induction of DNA double-strand breaks by components leached from dental composites in primary human gingival fibroblasts

Harry Scherthan Bernd Kaina Werner Geurtsen Juergen Durner Mohamed Shehata Lena Rothmund Kirsten Van Landuyt Franz X. Reichl Thomas Carell Reinhard Hickel Panorea Styllou Ayce Eldenez

subject

Programmed cell death Materials science Necrosis Cell Survival Cell Culture Techniques Gingiva Tetrazolium Salts Apoptosis medicine.disease_cause Composite Resins Cell Line Polyethylene Glycols Histones Dental Materials Necrosis Onium Compounds Organophosphorus Compounds Polymethacrylic Acids Materials Testing Stilbenes medicine Humans DNA Breaks Double-Stranded General Materials Science Viability assay Cytotoxicity General Dentistry Dose-Response Relationship Drug Biphenyl Compounds Fibroblasts Molecular biology Biphenyl compound Microscopy Fluorescence Mechanics of Materials Apoptosis Toxicity Methacrylates Indicators and Reagents medicine.symptom Genotoxicity Mutagens

description

Abstract Introduction The public interest steadily increases in the biological adverse effects caused by components released from resin-based dental restorations. Objective In this study, the cytotoxicity and the genotoxicity were investigated of following released components from dental resin restorations in human gingival fibroblasts (HGF): tetraethyleneglycol dimethacrylate (TEEGDMA), neopentylglycol dimethacrylate (Neopen), diphenyliodoniumchloride (DPIC), triphenyl-stibane (TPSB) and triphenylphosphane (TPP). Methods XTT based cell viability assay was used for cytotoxicity screening of substances. γ-H2AX assay was used for genotoxicity screening. In the γ-H2AX assay, HGFs were exposed to the substances for 6 h. Induced foci represent double DNA strand breaks (DSBs), which can induce ATM-dependent phosphorylation of the histone H2AX. Cell death effects (apoptosis and necrosis), induced by the substances were visually tested by the same investigator using the fluorescent microscope. Results All tested substances induced a dose-dependent loss of viability in HGFs. Following toxicity ranking among the substances at EC50-concentration were found in the XTT assay (mM, mean ± SEM; n = 5): DPIC > Neopen > TPSB > TPP > TEEGDMA. DSB-foci per HGF-cell were obtained, when HGFs were exposed to the EC50-concentration of each substance in the following order (mean ± SEM; n = 3): DPIC > Neopen > TPSB > TPP > TEEGDMA. Multi-foci cells (cells that contain more than 40 foci each) in 80 HGF-cells at EC50-concentration of each substance were found as follow (mean ± SEM; n = 3): DPIC > Neopen > TPP > TPSB > TEEGDMA. Cell apoptosis contained in each substance at EC50-concentration in the following order (mean ± SEM; n = 3): DPIC > Neopen > TPSB > TPP >TEEGDMA. Cell necrosis contained in each substance at EC50-concentration in the following order (mean ± SEM; n = 3): DPIC > Neopen > TPSB > TPP > TEEGDMA. Conclusion Leached components from dental resin restorations can induce DNA DSBs and cell death effects in HGFs.

year	journal	country	edition	language
2012-10-22	Dental Materials

https://doi.org/10.1016/j.dental.2013.07.007