6533b82cfe1ef96bd128fdd2

RESEARCH PRODUCT

Studying endocytosis in space and time by means of temporal Boolean models

Derek ToomreRafael SebastianM. E. DíazGuillermo AyalaRoberto ZoncuE. Diaz

subject

PhysicsSpacetimebiologyBoolean modelEndocytic cycleEndocytosisClathrinArtificial IntelligenceSignal ProcessingExtracellularbiology.proteinBiophysicsComputer Vision and Pattern RecognitionSoftwareIntracellular

description

Endocytosis is a process by which cells carry traffic from the extracellular space into various intracellular compartments. Visualization of fluorescently tagged clathrin proteins (mediators of endocytosis) allows us to image endocytosis in real time. When imaging the plasma membrane, areas of fluorescence generated by different endocytic processes overlap spatially and temporally, forming random clumps. Here, a sequence of segmented clathrin spots is considered a realization of a non-isotropic 3D Boolean model. Estimates of the intensity, the mean perimeter and the density function of the durations of endocytic events are obtained.

yearjournalcountryeditionlanguage
2006-11-01Pattern Recognition
https://doi.org/10.1016/j.patcog.2006.04.030