6533b82cfe1ef96bd1290762
RESEARCH PRODUCT
Intranasal immunization of mice with vp2 DNA of human rotavirus a induces cellular and humoral immunity.
F. Pourasgari S. Ahmadian Z. M. Hassan M. Mahdavi A. H. Salmanian M N P. Pothiersubject
[SDV.MP.VIR] Life Sciences [q-bio]/Microbiology and Parasitology/Virologyvirusesvirus diseasesbiochemical phenomena metabolism and nutrition[ SDV.MP.VIR ] Life Sciences [q-bio]/Microbiology and Parasitology/Virologydescription
International audience; DNA vaccination using a plasmid encoding Human rotavirus A (HuRV-A) inner capsid VP2 was examined in a mouse model. BALB/c mice were immunized intranasally (i.n.) with a VP2 DNA vaccine that induced cellular and humoral immune response to HuRV-A. The increased levels of cytokines IFN-gamma and IL-4 and the production of anti-VP2 IgG antibodies were detected. In addition, splenocyte proliferation detected by MTT test was enhanced in the mice treated with the vaccine. These results may encourage the development of a HuRV-A DNA vaccine derived from the inner layer of viral capsid that can be administered i.n. Key words: Human rotavirus A; VP2 protein; immunogenicity; DNA vaccine; mouse.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2008-01-01 |