6533b82dfe1ef96bd129095d

RESEARCH PRODUCT

Molecular Identification and Mycotoxin Production by Alternaria Species Occurring on Durum Wheat, Showing Black Point Symptoms

Antonia SuscaAntonio MorettiStefania SommaAntonio F. LogriecoStefano RavagliaMatteo FranzoniMario MasielloGiuseppe MecaVeronica Ghionna

subject

Health Toxicology and MutagenesisAlternariollcsh:MedicineToxicologyalternariol03 medical and health scienceschemistry.chemical_compoundspecies specific-primersGenotypesection <i>Alternaria</i>Grain qualityTenuazonic acidotorhinolaryngologic diseasesBlack pointsection <i>Infectoriae</i>Mycotoxin030304 developmental biology0303 health sciencesaltenuenebiology030306 microbiologylcsh:Rfood and beveragesAlternariabiology.organism_classificationalternariol-monomethyl etherHorticulturechemistrytenuazonic acidAlternaria species

description

Black point is a fungal disease of wheat, mainly associated with mycotoxigenic Alternaria species. Affected wheat kernels are characterized by dark brown discolouration of the embryo region and reduction of grain quality. Potential risk is the possible accumulation of Alternaria mycotoxins, alternariol (AOH), alternariol-monomethyl ether (AME), tenuazonic acid (TA), and altenuene (ALT), provided by haemato-toxic, genotoxic, and mutagenic activities. One hundred and twenty durum wheat samples belonging to 30 different genotypes grown in Bologna and Modena areas, in Italy, showing black point symptoms, were analyzed for Alternaria species and their mycotoxin contamination. Alternariol was selected as an indicator of the capability of the Alternaria species to produce mycotoxin in vivo in field conditions. The data showed that Alternaria species occurred in 118 out of 120 wheat kernels samples, with the incidence of infected kernels ranging between 1% and 26%. Moreover, AOH was detected by using a HPLC with a diode array detector (LC-DAD) in 98 out of 120 samples with values ranging between 24 and 262 &micro

yearjournalcountryeditionlanguage
2020-04-01Toxins
10.3390/toxins12040275http://dx.doi.org/10.3390/toxins12040275