6533b82dfe1ef96bd12909b0
RESEARCH PRODUCT
Face-selective adhesion of calcium oxalate dihydrate crystals to renal epithelial cells
S. DeganelloJohn C. LieskeF. G. Tobacksubject
Endocrinology Diabetes and MetabolismCrystallography X-RayKidneyEpitheliumCell LineCrystalKidney CalculiEndocrinologyChlorocebus aethiopsMonolayerCell AdhesionAnimalsOrthopedics and Sports MedicineKidney Tubules CollectingCalcium OxalateChemistryCell MembraneSubstrate (chemistry)AdhesionMolecular arrayKidney cellModels StructuralCrystallographyMembraneMicroscopy Electron ScanningCalcium oxalate dihydrate crystalsCrystallizationdescription
The interaction between the most common urinary crystal, calcium oxalate dihydrate (COD) and the surface of monkey renal epithelial cells of the BSC-1 line was investigated. The [100] face of exogenous COD crystals bound selectively and rapidly to the kidney cell surface. Cellular processes extended preferentially over the [100] face initially, and then progressively covered the crystal so that by 24 hours some crystals were observed beneath the plasma membrane. When nucleated from solution onto the surface of the cell monolayer, COD crystals oriented preferentially so that their [100] faces were in direct contact with the cell surface. In contrast, when siliconized glass was used as a substrate, nucleated COD crystals oriented randomly. Therefore, structures on the apical surface of renal tubular cells that mediate a stereospecific interaction with the molecular array presented by the [100] face of a COD crystal may be important determinants of crystal adhesion that could contribute to crystal retention and formation of kidney stones.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1996-03-01 | Calcified Tissue International |