6533b82dfe1ef96bd1290ba7
RESEARCH PRODUCT
CXCL10 and IL-6 induce chemotaxis in human trophoblast cell lines.
S. MartinezF. LoroJosé A. HorcajadasFrancisco DomínguezAlicia QuiñoneroCarlos SimónAntonio Pellicersubject
AdultEmbryologyChemokineReceptors CXCR3Protein Array AnalysisBiologyCXCR3Cell LineEndometriumCell MovementGeneticsmedicineCXCL10HumansRNA MessengerCXCL13Molecular BiologyMenstrual CycleInterleukin-6Reverse Transcriptase Polymerase Chain ReactionChemotaxisObstetrics and GynecologyTrophoblastChemotaxisCell BiologyImmunohistochemistryCell biologyTrophoblastsChemokine CXCL10medicine.anatomical_structureBlastocystReproductive MedicineCell cultureCulture Media Conditionedembryonic structuresImmunologybiology.proteinFemaleDevelopmental BiologyChemotaxis assaydescription
The investigation of trophoblast chemoattractive molecules in humans is of high interest for the reproductive field. Current evidence in ruminants demonstrates that CXCL10, formerly the interferon-gamma-inducible protein 10 (IP-10), is a potent chemotactic molecule implicated in the migration of trophoblast cells during early gestation. The aim of this work was to explore the existence of CXCL10/CXCR3 in the human model. Furthermore, chemotaxis assays were performed to demonstrate CXCL10 chemotactic activity in the human trophoblast cell lines JEG-3 and AC-1M88. Surprisingly, the conditioned media from epithelial endometrial cells (EEC) induced the highest trophoblast migration rate. Cytokine and chemokine membrane protein arrays were used to identify the secreted protein profile of EEC-conditioned media, and IL-6 was found to be the most abundant and CXCL13 the second most abundant molecule. Using a chemotaxis assay on AC-IM88, IL-6 antibody blocked the effect of EEC, indicating IL-6 to be an effective chemoattractive factor for trophoblast cells in the human model.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2008-05-20 | Molecular human reproduction |