6533b82dfe1ef96bd129153a

RESEARCH PRODUCT

Fast simultaneous determination of prominent polyphenols in vegetables and fruits by reversed phase liquid chromatography using a fused-core column

José Manuel Herrero-martínezMercedes ValcárcelJaime Cebolla-cornejoMiguel Valcárcel CasesSalvador RosellóRaúl Martí

subject

HPLC-DADAnalytical ChemistryFused corePhenolsResponse surface methodologyLimit of DetectionVegetablesResponse surface methodologyChromatography High Pressure LiquidFlavonoidsDetection limitChromatography Reverse-PhaseChromatographyChemistryExtraction (chemistry)Polyphenolsfood and beveragesGeneral MedicineReversed-phase chromatographyRepeatabilityGENETICAFunctional qualityPolyphenolFoodFruitUltrasound-assisted extractionResponse Surface MethodologyColumn (botany)Food Science

description

A reversed-phase high-performance liquid chromatography method with photodiode array detection has been developed enabling the joint determination of 17 prominent flavonoids and phenolic acids in vegetables and fruits. A multi-segmented gradient program using a fused-core column for the separation of several phenolic classes (phenolic acids and flavonoids) has been optimised. The influence of extraction conditions (sample freeze-drying, ultrasound extraction, solvent composition and extraction time) has been also optimised using response surface methodology with tomato samples as a model. Complete recoveries (76-108%) were obtained for the phenolic compounds present in tomato. The developed method provided satisfactory repeatability in terms of peak area (RSD < 2.9%) and retention time (RSD < 0.2%) both for standards and real samples. Detection limits ranged between 3 and 44 mu g kg(-1) for the detected polyphenols. This method is recommended for routine analysis of large number of samples typical of production quality systems or plant breeding programs.

yearjournalcountryeditionlanguage
2015-01-01
10.1016/j.foodchem.2014.07.151