6533b82dfe1ef96bd129167b
RESEARCH PRODUCT
Spectrophotometric and voltammetric determination of 2,5-dimethylaniline as potential product of enzymatic splitting of XY-2,5-dimethylanilide type substrates
subject
Dipeptidyl aminopeptidasesAmine determinationEnzyme activity determinationDiazo compoundsdescription
A method for determination of 2,5-dimethylaniline is proposed. The procedure enables both spectrophotometrical and voltammetric measurements. The spectrophotometrical determination at λmax = 385.8 nm is possible for concentrations up to 1.25 × 10-6 mol l-1. The voltammetric method takes more time, however, due to the adsorptive enrichment of the substrate it is possible to determine concentrations in the range of 10-7 mol l-1). In the paper the optimal conditions and parameters for the determination are suggested.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1999-01-01 | Chemia Analityczna |