6533b82efe1ef96bd1292782

RESEARCH PRODUCT

Diversity of chlorophenol-degrading bacteria isolated from contaminated boreal groundwater

Marja TiirolaJaakko A. PuhakkaMarkku S. KulomaaMinna K. MännistöMirja Salkinoja-salonen

subject

DNA BacterialPentachlorophenolfood.ingredientCaulobacterMolecular Sequence DataFresh WaterAquiferGram-Positive BacteriaBiochemistryMicrobiologyMicrobiology03 medical and health sciencesfoodRalstoniaRNA Ribosomal 16SGram-Negative BacteriaGeneticsMolecular BiologyPhylogeny030304 developmental biologyBase Composition0303 health sciencesgeographygeography.geographical_feature_categoryBacteriabiology030306 microbiologyPseudomonasNocardioidesGenetic VariationGeneral Medicinebiology.organism_classification6. Clean waterBiodegradation EnvironmentalEnvironmental chemistryWater MicrobiologyPolymorphism Restriction Fragment LengthWater Pollutants ChemicalBacteriaGroundwaterFlavobacteriumChlorophenols

description

Chlorophenol-degrading bacteria from a long-term polluted groundwater aquifer were characterized. All isolates degraded 2,4,6-trichlorophenol and 2,3,4,6-tetrachlorophenol at concentrations detected in the contaminated groundwater (10 mg 1(-1)). Pentachlorophenol was degraded by three isolates when present alone. In two gram-positive isolates, 2,3,4,6-tetrachlorophenol was required as an inducer for the degradation of pentachlorophenol. The gram-positive isolates were sensitive to pentachlorophenol, with an IC50 value of 5 mg/l. Isolates belonging to the Cytophaga/Flexibacter/Bacteroides phylum had IC50 values of 25 and 63 mg/l. Isolates belonging to alpha-, beta- and gamma-Proteobacteria generally tolerated the highest pentachlorophenol concentrations (100 mg/l). Polychlorophenol-degrading capacity was found in strains of Nocardioides, Pseudomonas, Ralstonia, Flavobacterium, and Caulobacter previously not known to degrade polychlorophenols. In addition, six polychlorophenol-degrading sphingomonads were found.

yearjournalcountryeditionlanguage
1999-02-22Archives of Microbiology
https://doi.org/10.1007/s002030050698