6533b82efe1ef96bd12929c1
RESEARCH PRODUCT
Cultivation of primmorphs from the marine sponge Suberites domuncula: morphogenetic potential of silicon and iron.
Renate SteffenMohammed Shokry A AmmarWerner E. G. M�llerFranz BrümmerVladislav A. GrebenjukSanja PerovicGaël Le Pennecsubject
IronCell Culture TechniquesBioengineeringApplied Microbiology and BiotechnologyBioreactorsSpecies SpecificityFood supplyMorphogenesisAnimalsGrowth SubstancesEcosystemCell AggregationPhylum PoriferabiologyEcologySilicatesGeneral MedicineExtracellular matrix moleculesbiology.organism_classificationCanal systemCell biologyPoriferaSuberites domunculaSpongeGene Expression RegulationFerritinsCell DivisionBiotechnologySuberitesdescription
Abstract Marine demosponges (phylum Porifera) are rich sources for potent bioactive compounds. With the establishment of the primmorph system from sponges, especially from Suberites domuncula , the technology to cultivate sponge cells in vitro improved considerably. This progress was possible after the elucidation that sponges are provided with characteristic metazoan cell adhesion receptors and extracellular matrix molecules which allow their cells a positioning in a complex organization pattern. This review summarizes recent data on the cultivation of sponges in aquaria and—with main emphasis—of primmorphs in vitro. It is outlined that silicon and Fe(+++) contribute substantially to the formation of larger primmorphs (size of 10 mm) as well as of a canal system in primmorphs; canals are probably required for an improved oxygen and food supply. We conclude that the primmorph system will facilitate a sustainable use of sponges in the production of bioactive compounds; it may furthermore allow new and hitherto not feasible insights into basic questions on the origin of Metazoa.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2002-11-09 | Journal of biotechnology |