6533b82efe1ef96bd129330c
RESEARCH PRODUCT
Initial steps of wall protoplast regeneration in Candida albicans
Carmen AguadoSalvador MormeneoRafael SentandreuHortensia RicoC. Carrillosubject
Time FactorsBlotting WesternChitinMicrobiologyMicrobiologyFungal ProteinsCell wallchemistry.chemical_compoundChitinCell WallCandida albicansCell Wall SkeletonFluorescent Antibody Technique IndirectCandida albicansGlucansMolecular BiologyGlucanchemistry.chemical_classificationMembrane GlycoproteinsbiologyProtoplastsRegeneration (biology)fungiGeneral MedicineProtoplastbiology.organism_classificationYeastcarbohydrates (lipids)Microscopy ElectronBiochemistrychemistryPolyclonal antibodiesbiology.proteinElectrophoresis Polyacrylamide GelCell Divisiondescription
Summary Cell wall regeneration of individual Candida albicans yeast and mycelial protoplasts was studied with confocal and electron microscopy using polyclonal antibodies and leetins. Quantitative measurements of the fluorescence emitted by individual protoplasts during the process of regeneration indicate that chitin is the first polymer to be laid down, whereas β(1,3)- and β(1,6)glucan are incorporated at a later stage. Mannoproteins were found on the surface of fresh protoplasts and those newly synthesized were then deposited with time. During the first steps of wall regeneration, the proteins that interacted covalently with chitin or glucan were different, but the same species were found linked to each polymer in yeast and mycelial regenerating forms. The aggregates formed by regenerating protoplasts were shown to be due to the chitin and mannoprotein network initially laid.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1997-09-01 | Research in Microbiology |