Characterization of particle morphology of biochanin A molecularly imprinted polymers and their properties as a potential sorbent for solid-phase extraction

6533b82efe1ef96bd129345a

RESEARCH PRODUCT

Characterization of particle morphology of biochanin A molecularly imprinted polymers and their properties as a potential sorbent for solid-phase extraction

Anna Poliwoda Piotr Wieczorek Anna M. Chrzanowska

subject

Acetonitriles Materials science Nitrogen Polymers Pyridines Ethylene glycol dimethacrylate Bioengineering Polymerization Biochanin A Biomaterials chemistry.chemical_compound Spectroscopy Fourier Transform Infrared solid-phase extraction Solid phase extraction Fourier transform infrared spectroscopy Chromatography High Pressure Liquid chemistry.chemical_classification phytoestrogens Acrylamide Chromatography Solid Phase Extraction Molecularly imprinted polymer Polymer Genistein Isoflavones Cross-Linking Reagents chemistry Methacrylic acid Mechanics of Materials adsorption Microscopy Electron Scanning Methacrylates molecular imprinting Molecular imprinting Nuclear chemistry

description

Abstract Molecularly imprinted polymers (MIPs) with biochanin A as a template were obtained using a bulk polymerization with non-covalent imprinting approach. The polymers were prepared in acetonitrile as porogen, using ethylene glycol dimethacrylate (EDMA) as cross-linking agent. The synthesis, with an application of 1′,1′-azobis(cyclohexanecarbonitrile) (ACHN) as an initiator, has been performed thermally. During the synthesis process the effect of different functional monomers such as methacrylic acid (MAA), acrylamide (AA) and 4-vinylpyridine (4-VP) was investigated. The application of nitrogen sorption porosimetry, scanning electron microscopy (SEM), and Fourier transform infrared spectroscopy (FTIR) permitted the characterization and evaluation of synthesized polymers. The adsorption capacity of obtained MIPs was checked by using the binding testing. All synthesized polymers were evaluated as solid-phase extraction (SPE) sorbents for isolation and preconcentration of biochanin A and its analogues, daidzein and genistein. The MIPs exhibited higher affinity for biochanin A over competitive compounds.

year	journal	country	edition	language
2014-10-07	Materials Science & Engineering C-Materials for Biological Applications

10.1016/j.msec.2015.01.069 https://doi.org/10.1016/j.msec.2015.01.069