6533b82efe1ef96bd1293be9

RESEARCH PRODUCT

Simple liquid chromatography assay for analyzing ochratoxin A in bovine milk

José Miguel SorianoJuan Carlos MoltóL. González-osnayaJordi Mañes

subject

Detection limitOchratoxin ABovine milkChromatographyExtraction (chemistry)food and beveragesGeneral MedicineContaminationAnalytical Chemistrylaw.inventionchemistry.chemical_compoundchemistrylawMycotoxinOchratoxinFiltrationFood Science

description

Abstract Ochratoxin A (OTA) is a mycotoxin with teratogenic and carcinogenic properties. Animal intake of feedstuffs contaminated with OTA may cause that some residues may be found in bovine milk, therefore, its analysis requires a highly sensitive, simple and precise technique. This method is based on a liquid–liquid extraction with methanol, followed by filtration and extract concentration. Liquid chromatography coupled to fluorescence detection was used for OTA analysis. In this way, several impurities are filtered off and OTA is quantified with a mean recovery of 93.0 ± 7.4% and a limit of detection of 0.01 ng mL −1 . Therefore, this methodology allows a simple quantitative extraction of OTA from bovine milk.

yearjournalcountryeditionlanguage
2008-05-01Food Chemistry
https://doi.org/10.1016/j.foodchem.2007.10.013