6533b82efe1ef96bd1293d64
RESEARCH PRODUCT
The 1.45 A resolution structure of the cryptogein-cholesterol complex: a close-up view of a sterol carrier protein (SCP) active site.
Michel PonchetMarie Bernard LascombePaul VenardThierry PrangéJean Pierre BleinMarie Louise Milatsubject
Models MolecularStereochemistryMolecular Sequence DataBiologyFungal Proteinschemistry.chemical_compoundStructural BiologyAmino Acid SequenceOomyceteBinding SitesMolecular StructureSequence Homology Amino AcidCholesterolPhytophthora cryptogeaResolution (electron density)Algal ProteinsActive siteGeneral Medicinebiology.organism_classificationSterolElicitorSterolsSterol carrier proteinCholesterolBiochemistrychemistrybiology.proteinCarrier Proteinsdescription
Cryptogein is a small 10 kDa elicitor produced by the phytoparasitic oomycete Phytophthora cryptogea. The protein also displays a sterol carrier activity. The native protein crystallizes in space group P4(1)22, with unit-cell parameters a = b = 46.51, c = 134.9 A (diffraction limit: 2.1 A). Its complex with cholesterol crystallizes in space group C222(1), with unit-cell parameters a = 30.96, b = 94.8, c = 65.3 A and a resolution enhanced to 1.45 A. The large inner non-specific hydrophobic cavity is able to accommodate a large variety of 3-beta-hydroxy sterols. Cryptogein probably acts as a sterol shuttle helping the pathogen to grow and complete its life cycle.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2002-02-17 | Acta crystallographica. Section D, Biological crystallography |