Penicillium lanoso-viride AMP dezamināzes attīrīšanas preparatīvo metožu izstrāde

6533b82efe1ef96bd1294080

RESEARCH PRODUCT

Penicillium lanoso-viride AMP dezamināzes attīrīšanas preparatīvo metožu izstrāde

Diāna Marcinkēviča

subject

Penicillium lanoso–viride AMP dezamināze divfāžu frakcionēšana Bioloģija proteīnu attīrīšana

description

AMP dezamināze ir glikoproteīns, kam piemīt imūnmodulējošas īpašības, tādēļ darba mērķis bija izstrādāt efektīvu un ātru AMPD attīrīšanas metodi no Penicillium lanoso–viride 8D, ar kuras palīdzību būtu iespējams iegūt proteīna preparātu ar pietiekami augstu AMPD aktivitāti. Izmantojot divfāžu frakcionēšanu ar PEG un amonija sulfātu, kam seko proteīnu precipitācija ar acetonu, tika atdalīts pigments un iegūts ekstrakts ar līdzīgu AMPD specifisko aktivitāti un lielāku aktivitātes iznākumu, kāds tiek iegūts, izmantojot proteīnu precipitāciju ar amonija sulfātu – līdz šim izmantoto metodi. AMPD attīrīšanai no koncentrētiem ekstraktiem tika izmantoti DEAE–Sephadex A–25, fosfoceluloze un konkanavalīna A sefaroze, tomēr ar to palīdzību neizdevās iegūt proteīnu preparātus ar augstu AMPD specifisko aktivitāti vai attīrīšanas pakāpi.

year	journal	country	edition	language
2015-01-01

https://dspace.lu.lv/dspace/handle/7/28582

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