Sējas kaņepju Cannabis sativa L. in vitro pavairošana un to ģenētiskās daudzveidības noteikšana

6533b82efe1ef96bd1294085

RESEARCH PRODUCT

Sējas kaņepju Cannabis sativa L. in vitro pavairošana un to ģenētiskās daudzveidības noteikšana

Karina Latiševa

subject

description

Sējas kaņepes (Cannabis sativa L.) ir lauksaimniecībā nozīmīgs kultūraugs. Jaunu šķirņu ātrākai iegūšanai ļoti nozīmīga ir in vitro un molekulāro metožu adaptācija un iekļaušana selekcijas shēmā, tādēļ šīm darbam ir sekojošs mērķis: sējas kaņepju in vitro pavairošanas un kultivēšanas metodes adoptēšana, augu ģenētiskas daudzveidības noteikšana, sieviešu dzimuma augu identfikācija izmantojot molekulārās metodes. Veicot molekulāri ģenētisko analīzi konstatēts, ka vietējas kaņepes ‘Puriņi’ un šķirnes ‘Finola’ kaņepes ir ģenētiski līdzīgas. Ģenētiska līdzība tika noteikta izmantojot uz PCR balstīto iPBS metodi. Sējas kaņepju dzimumu identificēšanai agrīnas stadijās ir piemērota SCAR (Sequence Characterized Amplified Region) metode. Augu in vitro kultivēšanai ir piemērota Murashige-Skoog barotne ar aktivēto ogli. Mātes augu klonēšanā in vitro labākos rezultātus var iegūt, pavairojot augus ar sānu dzinumiem.

year	journal	country	edition	language
2013-01-01

https://dspace.lu.lv/dspace/handle/7/20949

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