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RESEARCH PRODUCT

Ultrathin and nanostructured ZnO-based films for fluorescence biosensing applications

Yana AleevaMaria Elena FragalàCristina Satriano

subject

MOCVD–colloidal lithography; Protein adsorption; Fluorescence recovery after photobleachingMaterials scienceSilicon dioxideMOCVD-colloidal lithographyZnO thin film; MOCVD-colloidal lithography; Biosensing; Protein adsorption; Fluorescence recovery after photobleachingProtein adsorptionNanotechnologyBiointerfaceBiosensing TechniquesChemical vapor depositionFluorescenceFluorescence recovery after photobleachingBiomaterialschemistry.chemical_compoundColloid and Surface ChemistryAdsorptionX-ray photoelectron spectroscopyAlbuminschemistry.chemical_classificationBiosensingBiomoleculeMOCVD–colloidal lithographyMembranes ArtificialZnO thin filmSilicon DioxideNanostructuresSurfaces Coatings and FilmsElectronic Optical and Magnetic MaterialschemistryFluoresceinZinc OxideBiosensorProtein adsorption

description

The fluorescence-based sensing capability of ultrathin ZnO-SiO(2) nanoplatforms, deposited by an integrated approach of colloidal lithography and metal organic chemical vapor deposition, has been investigated upon adsorption of fluorescein-labeled albumin, used as model analyte biomolecule. The protein immobilization process after spontaneous adsorption/desorption significantly enhances the green emission of the different ZnO-based films, as evidenced by scanning confocal microscopy, corresponding to a comparable protein coverage detected by X-ray photoelectron spectroscopy. Moreover, experiments of fluorescence recovery after photobleaching evidence that the protein lateral diffusion at the biointerface is affected by the chemical and/or topographical patterning of hybrid ZnO-SiO(2) surfaces. The used approach is very promising for biomolecular detection applications of these ZnO-SiO(2) nanoplatforms, by simple sizing of the 2D vs. 3D patterning design, which in turn is accomplished by the fine tuning of the integrated colloidal lithography-chemical vapor deposition processes. (C) 2011 Elsevier Inc. All rights reserved.

yearjournalcountryeditionlanguage
2011-06-12Journal of Colloid and Interface Science
https://doi.org/10.1016/j.jcis.2011.09.014