6533b82ffe1ef96bd1295171

RESEARCH PRODUCT

Quantitative separation of Trichoderma lipid classes on a bonded phase column.

Y. HathoutMaurice BensoussanLeobardo Serrano-carreónJean-marc Belin

subject

chemistry.chemical_classificationTrichodermaChromatographyChromatographybiologyChemistryLinoleic acidTrichoderma virideFatty AcidsTrichoderma harzianumFatty acidGeneral ChemistryFungi imperfectibiology.organism_classificationLipidschemistry.chemical_compoundColumn chromatographyLinoleic AcidsPhase (matter)Trichodermalipids (amino acids peptides and proteins)Phospholipids

description

Bond Elut aminopropyl columns were used to purify the different lipid classes of Trichoderma harzianum and Trichoderma viride. This methodology permitted good separation of the fungal lipid classes in less time than traditional techniques. The incorporation of [1 (14)C]linoleic acid into neutral lipids, free fatty acids and phospholipids was quantified for both strains. The fatty acid profile of the different lipid classes of these fungal strains was determined as a function of culture time.

yearjournalcountryeditionlanguage
1992-12-01Journal of chromatography
10.1016/0378-4347(92)80018-lhttps://pubmed.ncbi.nlm.nih.gov/1487513