6533b82ffe1ef96bd129647c

RESEARCH PRODUCT

Evaluation of advanced silica packings for the separation of biopolymers by high-performance liquid chromatography

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Abstract In the separation of proteins and peptides by the various modes of high-performance liquid chromatography, the nature of the substrates requires the use of microparticulate silica packings with bonded ligands of appropriate design. Agglomeration of monodisperse silica hydrosols of defined size distribution into beaded particles provides a useful method of controlling the pore size, the size distribution, the particle porosity and surface area of these packings. The particle porosity is shown to be a major factor governing the packing density and packing stability of the column. For size-exclusion chromatography of proteins, parent silicas of pore size 10 and 100 nm with a narrow pore size distribution, adequate particle porosity and low surface area should be employed for appropriate bonding, whereas reversed-phase and ion-exchange chromatography require pore sizes of 30–50 nm with specific surface areas between 50 and 100 m 2 g −1 and particle porosities of ⩽-50%