6533b82ffe1ef96bd12964df
RESEARCH PRODUCT
Porous silicon photoluminescence biosensor for rapid and sensitive detection of toxins
Roman ViterIgor IatsunskyiValerii MyndrulKaryna PavlovaValentyn SmyntynaYulia Melnyksubject
Ochratoxin AChromatographyPhotoluminescenceMaterials science010401 analytical chemistrytechnology industry and agricultureAnalytical chemistry02 engineering and technologyOptical biosensor021001 nanoscience & nanotechnologyPorous silicon01 natural sciencesIsotropic etching0104 chemical scienceschemistry.chemical_compoundchemistry0210 nano-technologyBiosensorVolume concentrationdescription
A rapid and low cost photoluminescence (PL) immunosensor for the determination of low concentrations of Ochratoxin A(OTA) and Aflatoxine B1 (AfB1) has been developed. This biosensor was based on porous silicon (PSi) fabricated by metal-assisted chemical etching (MACE) and modified by antibodies against OTA/AfB1 (anti-OTA/anti-AfB1). Biofunctionalization method of the PSi surface by anti-OTA/ anti-AfB1 was developed. The changes of the PL intensity after interaction of the immobilized anti-OTA/anti-AfB1with OTA/AfB1 antigens were used as biosensor signal, allowing sensitive and selective detection of OTA/AfB1 antigens in BSA solution. The sensitivity of the reported optical biosensor towards OTA/AfB1 antigens is in the range from 10 -3 to 10 2 ng/ml.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2017-08-25 | Organic Sensors and Bioelectronics X |