6533b830fe1ef96bd1296727

RESEARCH PRODUCT

Presence and coding properties of 2'-O-methyl-5-carbamoylmethyluridine (ncm5Um) in the wobble position of the anticodon of tRNA(Leu) (U*AA) from brewer's yeast.

Jean DesgresGérard KeithAnne-lise GlasserManuel A. S. SantosChakib El AdlouniMick F. TuiteElzbieta SochackaAndrzej Malkiewicz

subject

IdentificationRNA Transfer LeuStereochemistryBiophysicsAminoacylationWobble base pairModified nucleosideSaccharomyces cerevisiaeBiochemistryMass SpectrometryFungal Proteinschemistry.chemical_compoundStructural BiologyGeneticsAnticodonMolecular BiologyUridineChromatography High Pressure Liquidchemistry.chemical_classificationMolecular StructureRNA FungalCell BiologyUridineYeastYeastEnzymechemistryBiochemistryTransfer RNAtRNALeu (U*AA)Spectrophotometry UltravioletLeucineNucleoside

description

AbstractThe unknown modified nucleoside U* has been isolated by enzymatic and HPLC protocols from tRNALeu(U*AA) recently discovered in brewer's yeast. The pure U* nucleoside has been characterized by electron impact mass spectroscopy, and comparison of its chromatographic and UV-absorption properties with those of appropriate synthetic compounds. The structure of U* was established as 2′-O-methyl-5-carbamoylmethyluridine (ncm5Um). The yeast tRNALeu (U*AA) is the only tRNA so far sequenced which has been shown to contain ncm5Um. The location of such a modified uridine at the first position of the anticodon restricts the decoding property to A of the leucine UUA codon.

yearjournalcountryeditionlanguage
1992-12-21FEBS letters
10.1016/0014-5793(92)81510-shttps://pubmed.ncbi.nlm.nih.gov/1468572