6533b830fe1ef96bd1296768

RESEARCH PRODUCT

Plasmid localisation of atrazine-degrading genes in newly described Chelatobacter and Arthrobacter strains

Alain HartmannGuy SoulasSandrine Rousseaux

subject

GeneticsTransposable element0303 health sciencesEcologybiology030306 microbiologyBIOLOGIE MOLECULAIREbiology.organism_classificationApplied Microbiology and BiotechnologyMicrobiologyArthrobacter crystallopoietesMicrobiologylaw.invention03 medical and health sciencesPlasmid[SDV.MP]Life Sciences [q-bio]/Microbiology and ParasitologylawArthrobacterInsertion sequence[SDV.MP] Life Sciences [q-bio]/Microbiology and ParasitologyGeneBacteriaPolymerase chain reactionComputingMilieux_MISCELLANEOUS030304 developmental biology

description

Abstract In a previous study, we isolated a collection of atrazine-degrading bacteria from various soils. The aim of this study was to localise the atrazine-degrading genes in these 25 atrazine-degrading strains. In the case of the Gram-negative strains of Chelatobacter heintzii, six to seven plasmids were observed. The atzABC and trzD genes were located on two or three plasmids with variable molecular masses. For the Gram-positive strains of Arthrobacter crystallopoietes, the atzBC genes were located on a single plasmid of 117 kb. The organisation of atrazine-degrading genes seems to be highly variable between the strains studied. We have shown by a specific PCR the occurrence of IS1071-like sequences in all strains carrying atzBC genes, while these sequences seem to be absent in strains carrying only the atzA gene. Thus, we show a strong correlation between the presence of these insertion sequences and the presence of some genes involved in atrazine degradation.

yearjournalcountryeditionlanguage
2002-01-01
https://hal.inrae.fr/hal-02671838