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RESEARCH PRODUCT
Significance of gamma-glutamyltranspeptidase in exocrine pancreatic amino acid transport.
Giovanni E. MannK. DoolabhJ. SastreJose ViñaJ.h. Sweirysubject
Malemedicine.medical_specialtyGlutamineBiophysicsIndicator Dilution TechniquesBiologydigestive systemBiochemistryEpitheliumchemistry.chemical_compoundIn vivoInternal medicineNeutral amino acid transportmedicineAnimalsAmino AcidsAcivicinPancreasEpithelial polaritychemistry.chemical_classificationAlanineBiological TransportRats Inbred StrainsCell BiologyIsoxazolesgamma-Glutamyltransferasedigestive system diseasesEpitheliumAmino acidRatsEnzymemedicine.anatomical_structureEndocrinologychemistryPancreasdescription
The exocrine pancreas is rich in gamma-glutamyltranspeptidase (GGT, EC 2.3.2.2) and exhibits high rates of amino acid transport and protein synthesis. The role of the gamma-glutamyl cycle in mediating neutral amino acid transport in the isolated perfused rat pancreas was investigated using acivicin, an inhibitor of GGT, and a rapid dual isotope dilution technique. When treatment in vivo with acivicin (50 mg/kg) was followed 1 h later by continuous perfusion of the isolated pancreas with 10 microM acivicin, GGT levels decreased from 53 +/- 3 IU/g to 4.9 +/- 1.5 IU/g. This marked inhibition of GGT activity was not associated with decreased uptake for either L-alanine or L-glutamine, suggesting that the gamma-glutamyl cycle plays a negligible role in amino acid transport across the basolateral membrane of the pancreatic epithelium.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1991-06-18 | Biochimica et biophysica acta |