Izolētu un kultivētu hloroplastu morfoloģiskais un funkcionālais raksturojums

6533b830fe1ef96bd1296a4d

RESEARCH PRODUCT

Izolētu un kultivētu hloroplastu morfoloģiskais un funkcionālais raksturojums

Daiga Krieviņa

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Hloroplasti - proteīnu sintēzei un enerģijas transformācijas pētījumiem lietojama struktūra. Mērķis: izvērtēt izolētu, kultivētu hloroplastu morfoloģiskās izmaiņas, novērtēt to spēju sintezēt rekombinantus proteīnus. Darbā izmantoja konfokālā lāzerskenējošā un gaišā lauka mikroskopijas, SEM, programmas: “Image J” un “LAS AF Lite”, vektorus ar GFP. Izolētā hloroplastu kultūrā bija trīs morfoloģiski un funkcionāli atšķirīgas grupas. Tajās vēroja granu struktūras, fluorescences un šķērsgriezuma laukuma izmaiņas, autofāgijas procesus, dalīšanās un saplūšanas iezīmes. Transformētas plastīdas importēja nukleīnskābes un ekspresēja rekombinantos proteīnus. Maģistra darbs izstrādāts LU Bioloģijas fakultātes augu šūnu laboratorijā no 2012. gada līdz 2014. gadam. Atslēgas vārdi: hloroplasti, konfokālā mikroskopija, hlorofila fluorescence, SEM.

year	journal	country	edition	language
2014-01-01

https://dspace.lu.lv/dspace/handle/7/20985

PEPhysical Sciences and Engineering
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