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RESEARCH PRODUCT
Chapter 8 Native and Bonded Silicas in Aqueous Sec
J.n. KinkelK.k. Ungersubject
Pore sizeChromatographyAqueous solutionVolume (thermodynamics)ElutionChemistrySupport designSize-exclusion chromatographyHigh resolutionSurface modificationdescription
Publisher Summary This chapter deals with the properties of packings and columns in correlation to chromatographic separations in size exclusion chromatography (SEC) and provides guidelines for optimum support design. The chapter provides a survey of the current state-of-the-art with respect to the surface modification of silica for SEC packings in aqueous eluents. The properties of commercial packings and columns are listed and compared. An advice is also discussed on the operation and maintenance of columns with a view to achieving high resolution and long life-time. In SEC, solute elution is based on equilibrium, entropy-controlled size exclusion process. According to their size, the solutes penetrate the accessible volume of the pores of the packing. The elution volume V e ranges from V 0 (the interparticle column volume in the case of a totally excluded solute) to V 0 + V i for a totally permeating solute. V i corresponds to the total pore volume of the packing, termed intraparticle column volume. On account of their size, small solutes will migrate through a larger portion of V i at a given pore size and will be eluted later than larger solutes.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1988-01-01 |