6533b830fe1ef96bd1297101
RESEARCH PRODUCT
Digital image processing for rapid analysis of differentially expressed transcripts on high-density cDNA arrays.
Boris AdryanVolker CarlguthHans Jochen Deckersubject
DNA ComplementaryCDNA ArraysTranscription Geneticbusiness.industryHigh densityColorGene ExpressionComputational biologyVisual identificationBiologyBioinformaticsGeneral Biochemistry Genetics and Molecular BiologySet (abstract data type)SoftwareFilter (video)Complementary DNADigital image processingImage Processing Computer-AssistedAutoradiographyCloning MolecularbusinessSoftwareBiotechnologyDensitometrydescription
Usage of filter arrays is becoming increasingly attractive for many research laboratories involved in determination of gene-expression profiles. However, analysis of numerous spots, representing genes or partial gene sequences (ESTs), is still tedious work involving the ordered analysis of vast amounts of numerical tabular data. We present a rapid and efficient method for the visual identification of differentially expressed targets on high-density cDNA filter arrays using standard laboratory equipment and standard software, which is available for free. The method we introduce provides an inexpensive alternative, and no changes in the experimental set up are required. Our results were verified by densitometric analyses performed with an established system.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1999-06-01 | BioTechniques |